Fentanyl Laboratory Testing Cutoffs and Methods

The following is an explanation of the fentanyl screen and confirmation procedures/cutoff levels. The cutoff levels listed below may periodically change.

2101: Urine Fentanyl Screening Panel
Drug Screening Panel Methodology Cutoff
Level
Fentanyl EIA 1 ng/mL


5504: Urine Fentanyl Confirmation Panel
Drug Screening Panel Methodology Cutoff
Level
Fentanyl LC-MS/MS 0.5 ng/mL
Acetyl fentanyl LC-MS/MS 0.5 ng/mL
Norfentanyl LC-MS/MS 0.5 ng/mL
Methoxyacetyl fentanyl LC-MS/MS 0.5 ng/mL
Tetrahydrofuranyl fentanyl LC-MS/MS 0.5 ng/mL
Acryl fentanyl LC-MS/MS 0.5 ng/mL
Fluorofentanyl LC-MS/MS 0.5 ng/mL
Furanyl fentanyl LC-MS/MS 0.5 ng/mL
Isobutyryl fentanyl LC-MS/MS 0.5 ng/mL
Butyryl fentanyl LC-MS/MS 0.5 ng/mL
Valeryl fentanyl LC-MS/MS 0.5 ng/mL


5554: Urine Premium Fentanyl Confirmation Panel
Drug Screening Panel Methodology Cutoff
Level
4-ANPP LC-MS/MS 1.0 ng/mL
4'-Methylacetyl fentanyl LC-MS/MS 0.5 ng/mL
Acetyl fentanyl LC-MS/MS 0.5 ng/mL
Acetyl norfentanyl LC-MS/MS 5.0 ng/mL
Acryl fentanyl LC-MS/MS 0.5 ng/mL
Alfentanyl LC-MS/MS 0.5 ng/mL
alpha-Methylacetyl fentanyl LC-MS/MS 0.5 ng/mL
alpha-Methyl fentanyl LC-MS/MS 0.5 ng/mL
beta-Hydroxythio fentanyl LC-MS/MS 0.5 ng/mL
Butyryl fentanyl LC-MS/MS 0.5 ng/mL
Butyryl norfentanyl LC-MS/MS 0.5 ng/mL
Carfentanil LC-MS/MS 0.5 ng/mL
cis-3-Methyl fentanyl LC-MS/MS 0.5 ng/mL
Cyclopentyl fentanyl LC-MS/MS 0.5 ng/mL
Cyclopropyl fentanyl LC-MS/MS 0.5 ng/mL
para-Fluorobutyryl fentanyl LC-MS/MS 0.5 ng/mL
Fentanyl LC-MS/MS 0.5 ng/mL
Fluoroisobutyryl fentanyl LC-MS/MS 0.5 ng/mL
Fluoro fentanyl LC-MS/MS 0.5 ng/mL
Furanyl fentanyl LC-MS/MS 0.5 ng/mL
Furanyl norfentanyl LC-MS/MS 5.0 ng/mL
Isobutyryl fentanyl LC-MS/MS 0.5 ng/mL
Methoxyacetyl fentanyl LC-MS/MS 0.5 ng/mL
Norfentanyl LC-MS/MS 0.5 ng/mL
Ocfentanil LC-MS/MS 0.5 ng/mL
para-Chloroisobutyryl fentanyl LC-MS/MS 0.5 ng/mL
para-Methoxybutyryl fentanyl LC-MS/MS 0.5 ng/mL
Tetrahydrofuranyl fentanyl LC-MS/MS 0.5 ng/mL
Valeryl fentanyl LC-MS/MS 0.5 ng/mL


F901: Oral Fluid Fentanyl Screen and Confirmation Panel
Fentanyl Screening Panel Methodology Cutoff
Level
Fentanyl EIA 5 ng/mL

Fentanyl Confirmation Panel Methodology Cutoff
Level
Fentanyl
Acetylfentanyl
Acrylfentanyl
Butyrylfentanyl
Furanylfentanyl
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
1 ng/mL
1 ng/mL
1 ng/mL
1 ng/mL
1 ng/mL


The Resource Library includes information available in our printed Reference Guide, delivered to all new clients. To download a PDF of our Reference Guide, click here.