Fentanyl Laboratory Testing Cutoffs and Methods
The following is an explanation of the fentanyl screen and confirmation procedures/cutoff levels. The cutoff levels listed below may periodically change.
| 2101: Urine Fentanyl Screening Panel | ||
|---|---|---|
| Drug Screening Panel | Methodology | Cutoff Level |
| Fentanyl | EIA | 1 ng/mL or 2 ng/mL, depending on agency |
| 5504: Urine Fentanyl Confirmation Panel | ||
|---|---|---|
| Drug Screening Panel | Methodology | Cutoff Level |
| Fentanyl | LC-MS/MS | 0.5 ng/mL |
| Acetyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Norfentanyl | LC-MS/MS | 0.5 ng/mL |
| Methoxyacetyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Tetrahydrofuranyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Acryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Fluorofentanyl | LC-MS/MS | 0.5 ng/mL |
| Furanyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Isobutyryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Butyryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Valeryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| 5554: Urine Premium Fentanyl Confirmation Panel | ||
|---|---|---|
| Drug Screening Panel | Methodology | Cutoff Level |
| 4-ANPP | LC-MS/MS | 1.0 ng/mL |
| 4'-Methylacetyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Acetyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Acetyl norfentanyl | LC-MS/MS | 5.0 ng/mL |
| Acryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Alfentanyl | LC-MS/MS | 0.5 ng/mL |
| alpha-Methylacetyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| alpha-Methyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| beta-Hydroxythio fentanyl | LC-MS/MS | 0.5 ng/mL |
| Butyryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Butyryl norfentanyl | LC-MS/MS | 0.5 ng/mL |
| Carfentanil | LC-MS/MS | 0.5 ng/mL |
| cis-3-Methyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Cyclopentyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Cyclopropyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| para-Fluorobutyryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Fentanyl | LC-MS/MS | 0.5 ng/mL |
| Fluoroisobutyryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Fluoro fentanyl | LC-MS/MS | 0.5 ng/mL |
| Furanyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Furanyl norfentanyl | LC-MS/MS | 5.0 ng/mL |
| Isobutyryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Methoxyacetyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Norfentanyl | LC-MS/MS | 0.5 ng/mL |
| Ocfentanil | LC-MS/MS | 0.5 ng/mL |
| para-Chloroisobutyryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| para-Methoxybutyryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Tetrahydrofuranyl fentanyl | LC-MS/MS | 0.5 ng/mL |
| Valeryl fentanyl | LC-MS/MS | 0.5 ng/mL |
| F901: Oral Fluid Fentanyl Screen and Confirmation Panel | ||
|---|---|---|
| Fentanyl Screening Panel | Methodology | Cutoff Level |
| Fentanyl | EIA | 5 ng/mL |
| Fentanyl Confirmation Panel | Methodology | Cutoff Level |
| Fentanyl Acetylfentanyl Acrylfentanyl Butyrylfentanyl Furanylfentanyl |
LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS |
1 ng/mL 1 ng/mL 1 ng/mL 1 ng/mL 1 ng/mL |
How to read your Toxicology Report »
Non-Clinical Test Requisition Form video »