Laboratory Testing Cutoffs & Methods

The following is an explanation of RTL's urine screening and confirmation procedures/cutoff levels. The routine cutoff levels listed below may periodically change. The analytical methods used by RTL are scientifically accepted and approved by the U.S. Department of Health and Human Services (CMS / CLIA).


Comprehensive Panel Screen

The following list provides screening detail for the Comprehensive Drug Test panel. RTL screens urine specimens by enzyme assay (EA), enzyme immunoassay (EIA), gas chromatography-mass spectrometry (GC-MS). Presumptive positive specimens will be confirmed by a second method (GC-MS) and/or liquid chromatography-tandem mass spectrometry (LC-MS/MS) prior to reporting positive results.

Screen Reporting Levels By Procedure

Drug Method Cutoff
Alcohol (Ethanol) EA 0.04 g/dL
Amphetamines
  - Amphetamines
  - Methylenedioxyamphetamine (MDA)
  - Methylenedioxymethamphetamine (MDMA)
  - Methylenedioxyethylamphetamine (MDEA)
 
EIA
LC-MS/MS
LC-MS/MS
LC-MS/MS
 
1000 ng/mL
250 ng/mL
250 ng/mL
250 ng/mL
Anticonvulsants
  - Carbamazepine and/or Metabolite
  - Oxcarbazepine Metabolite
 
LC-MS/MS
LC-MS/MS
 
100 ng/mL
100 ng/mL
Antidepressants
  - Amitriptyline/Nortriptyline
  - Bupropion Metabolite
  - Citalopram/Escitalopram
  - Desipramine/Imipramine
  - Doxepin
  - Fluoxetine and/or Metabolite
  - Maprotiline
  - Paroxetine
  - Sertraline
  - Trazodone/Nefazodone and/or Metabolite
  - Venlafaxine Metabolite
 
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
 
100 ng/mL
250 ng/mL
150 ng/mL
100 ng/mL
100 ng/mL
150 ng/mL
100 ng/mL
150 ng/mL
150 ng/mL
Qualitative
100 ng/mL
Barbiturates EIA 200 ng/mL
Benzodiazepines
  - Alprazolam Metabolite
  - Clonazepam Metabolite
  - Flunitrazepam Metabolite
  - Flurazepam Metabolite
  - Lorazepam
  - Midazolam Metabolite
  - Nordiazepam
  - Oxazepam
  - Temazepam
  - Triazolam Metabolite
 
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
 
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
Cocaine EIA 300 ng/mL
Methylphenidate LC-MS/MS 50 ng/mL
Narcotics/Opiates
  - Buprenorphine
  - Butorphanol Metabolite
  - Codeine
  - Fentanyl
  - Hydrocodone
  - Hydromorphone
  - Ketamine Metabolite
  - Meperidine Metabolite
  - Methadone
  - Morphine
  - Nalbuphine
  - Oxycodone/Oxymorphone
  - Pentazocine
  - Propoxyphene
  - Tramadol and/or Metabolite
 
EIA
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
EIA
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
EIA
LC-MS/MS
 
5 ng/mL
Qualitative
100 ng/mL
5 ng/mL
100 ng/mL
100 ng/mL
25 ng/mL
25 ng/mL
150 ng/mL
100 ng/mL
100 ng/mL
50 ng/mL
100 ng/mL
300 ng/mL
100 ng/mL
Phencyclidine (PCP) EIA 25 ng/mL
Sedative/Hypnotic Agents
  - Carisoprodol/Meprobamate
  - Zolpidem Metabolite

LC-MS/MS
LC-MS/MS

200 ng/mL
10 ng/mL
THC (Marijuana) EIA 50 ng/mL


Comprehensive Panel Confirmation

The following list provides confirmation detail for the Comprehensive Drug Test panel. RTL confirms urine specimens by gas chromatography-mass spectrometry (GC-MS), liquid chromatography-tandem mass spectrometry (LC-MS/MS) and/or gas chromatography-flame ionization detector (GC-FID). The analytical methods used by RTL are scientifically accepted.

Confirmation Cutoff Levels By Procedure

Drug Method Cutoff
Alcohol (Ethanol) GC-FID 0.02 g/dL
Amphetamines
  - Amphetamine
  - Methamphetamine
  - Methylenedioxyamphetamine (MDA)
  - Methylenedioxymethamphetamine (MDMA)
  - Methylenedioxyethylamphetamine (MDEA)

LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS

250 ng/mL
250 ng/mL
250 ng/mL
250 ng/mL
250 ng/mL
Anticonvulsants
  - Carbamazepine
  - 10,11-Dihydroxycarbazepine
  - Oxcarbazepine
  - 10-Hydroxycarbazepine
 
GC-MS
GC-MS
GC-MS
GC-MS
 
100 ng/mL
Qualitative
100 ng/mL
100 ng/mL
Antidepressants
  - Amitriptyline
  - Nortriptyline
  - Hydroxybupropion
  - Citalopram/Escitalopram
  - Desipramine
  - Imipramine
  - Doxepin
  - Desmethyldoxepin
  - Fluoxetine
  - Norfluoxetine
  - Maprotiline
  - Paroxetine
  - Sertraline
  - Norsertraline
  - mCPP
  - Hydroxy-mCPP
  - Trazodone
  - Hydroxytrazodone
  - Venlafaxine
  - O-Desmethylvenlafaxine
 
LC-MS/MS
LC-MS/MS
GC-MS
GC-MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
GC-MS
GC-MS
GC-MS
GC-MS
GC-MS
GC-MS
GC-MS
GC-MS
GC-MS
GC-MS
LC-MS/MS
LC-MS/MS
 
25 ng/mL
25 ng/mL
250 ng/mL
150 ng/mL
25 ng/mL
25 ng/mL
25 ng/mL
25 ng/mL
150 ng/mL
150 ng/mL
100 ng/mL
150 ng/mL
150 ng/mL
150 ng/mL
100 ng/mL
Qualitative
Qualitative
Qualitative
100 ng/mL
100 ng/mL
Barbiturates
  - Amobarbital
  - Butabarbital
  - Butalbital
  - Pentobarbital
  - Phenobarbital
  - Secobarbital

GC-MS
GC-MS
GC-MS
GC-MS
GC-MS
GC-MS
 
200 ng/mL
200 ng/mL
200 ng/mL
200 ng/mL
200 ng/mL
200 ng/mL
Benzodiazepines
  - alpha-Hydroxyalprazolam (Alprazolam)
  - 7-Aminoclonazepam (Clonazepam)
  - 7-Aminoflunitrazepam (Flunitrazepam)
  - 2-Hydroxyethyl flurazepam (Flurazepam)
  - Lorazepam
  - alpha-Hydroxymidazolam (Midazolam)
  - Nordiazepam
  - Oxazepam
  - Temazepam
  - alpha-Hydroxytriazolam (Triazolam)
 
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
 
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
Cocaine LC-MS/MS 100 ng/mL
Methylphenidate GC-MS Qualitative
Narcotics/Opiates
  - Buprenorphine
  - Norbuprenorphine
  - Butorphanol
  - Hydroxybutorphanol
  - Codeine
  - Fentanyl
  - Hydrocodone
  - Hydromorphone
  - Ketamine
  - Norketamine
  - Dehydronorketamine
  - Normeperidine
  - Methadone
  - Methadone Metabolite (EDDP)
  - Morphine
  - Nalbuphine
  - Noroxycodone
  - Oxycodone
  - Oxymorphone
  - Pentazocine
  - Propoxyphene
  - Norpropoxyphene
  - Tramadol
  - O-Desmethyl-cis-Tramadol
 
LC-MS/MS
LC-MS/MS
GC-MS
GC-MS
LC-MS/MS
GC-MS
LC-MS/MS
LC-MS/MS
GC-MS
GC-MS
GC-MS
GC-MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
GC-MS
LC-MS/MS
LC-MS/MS
LC-MS/MS
GC-MS
GC-MS
GC-MS
LC-MS/MS
LC-MS/MS
 
0.5 ng/mL
2 ng/mL
5 ng/mL
Qualitative
100 ng/mL
5 ng/mL
100 ng/mL
100 ng/mL
5 ng/mL
5 ng/mL
25 ng/mL
50 ng/mL
100 ng/ml
100 ng/mL
100 ng/mL
250 ng/mL
50 ng/mL
50 ng/mL
50 ng/mL
250 ng/mL
200 ng/mL
200 ng/mL
100 ng/mL
100 ng/mL
Phencyclidine (PCP) GC-MS 5 ng/mL
Sedative/Hypnotic Agents
  - Carisoprodol
  - Meprobamate
  - Zolpidem
  - Carboxyzolpidem

LC-MS/MS
LC-MS/MS
LC-MS/MS
LC-MS/MS

100 ng/mL
100 ng/mL
1 ng/mL
10 ng/mL
THC (Marijuana)
  - THC-COOH (Marijuana)
  - THC-COOH/Creatinine Ratio

LC-MS/MS
Calculation

5 ng/mL


Urinalysis Adulteration Examination

RTL's Standard Operating Procedures (SOP's) require that each urine specimen be examined for signs of adulteration. This examination includes the following



To learn more about RTL's laboratory drug tests call:

800.255.2159

The Resource Library includes information available in our printed Reference Guide, delivered to all new clients. To download a PDF of our Reference Guide, click here.